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. 2015 Aug 3;210(3):471–483. doi: 10.1083/jcb.201411033

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© 2015 Lin et al.

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Figure 1. — Lrrk localizes at GOPs in dendrites. (A) Lrrk mutant alleles (top); e03680 insertion and del6 allele shown by dashed line. Lrrk domains (bottom): ANK, ankyrin repeats; LRR, leucine-rich repeats; ROC, Ras of complex; COR, C-terminal of ROC; Kin, S/T kinase; and WD, WD40 repeats. (B) Lrrk expressions in different genotypes were detected at 240 kD by Lrrk antibodies and control by α-Tubulin (α-Tub). The experiment was repeated three times. (C) Enriched Lrrk signal (magenta) localizes to ManII-GFP (green) expressed by ppk-GAL4. Enriched signal is absent in e03680/del6 (bottom). HRP is in blue in merged images. (D) Transgenic YFP-Lrrk (green) expressed by ppk-GAL4 shows puncta in mCD8-RFP-labeled neurons (red). (E) Cell bodies show colocalized YFP-Lrrk– (magenta) and ManII-GFP (green)–labeled puncta. (F) Dendrites labeled by mCD8-RFP (blue) show colocalization of YFP-Lrrk (magenta) and ManII-GFP (green). Arrow, arrowhead, and asterisk denote puncta at dendritic shaft, branch point, and tip, respectively. Bars, 10 µm.