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. 2015 Jul 19;88(6):561–572. doi: 10.1007/s11103-015-0342-x

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Comparison of gRNA expression cassettes. a RCs in pZH_Cas9 and pZK_OsU3-gYSA or pZK_OsU6-gYSA transformed calli. pZK_OsU3-gYSA and pZK_OsU6-gYSA were transformed into the same clonally propagated transgenic callus of pZH_Cas9. DNAs extracted from double transformed calli of pZH_Cas9 and pZK_OsU3-gYSA or pZK_OsU6-gYSA were subjected to PCR and subsequent SfiI restriction enzyme digestion. Blue bars show the average RC in independent Cas9 transgenic lines #1–#4 (or #3) transformed with pZK_OsU3-gYSA. Green bars show the average RC in independent Cas9 transgenic lines #1–#4 (or #3) transformed with pZK_OsU6-gYSA. b, c CAPS analysis of the gYSA locus in MMomegaCas9, AtomegaCas9 line #1 transformed with pZK_OsU3-gYSA or pZK_OsU6-gYSA. HMFs in pZH_Cas9 and pZK_OsU3-gYSA or pZK_OsU6-gYSA double transformed calli are indicated by red rectangles