Fig. 5.

Targeted mutagenesis using pZH_OsU6gRNA_MMCas9 vector. a Construction of pZH_OsU6gRNA_MMCas9 vector. b CAPS analysis of the gPDS-1 target locus. DNAs extracted from independent pZH_OsU6gPDS-1_MMCas9 transformed calli were subjected to PCR and subsequent PstI restriction enzyme digestion. WT non-transgenic callus lines. The HMF score is shown under the red rectangle. Mutation frequency in the other line #5 is shown under the yellow rectangle. c Mutations detected in lines #5 and #8