Fig. 6.

Targeted mutagenesis in the rice DL gene. a Target sites of CRISPR/Cas9-mediated target mutagenesis in the rice DL gene. gDL-1, 2, 3, 4 are located on exons 2 and 3 and intron 2, respectively. b CAPS analysis of the gDL-3 target locus in pZH_OsU6gDL-3_MMCas9-transformed calli. In the 7 transgenic lines analyzed (#1–7), few cleaved PCR products were detected. Mutation frequency in callus line #3 estimated by sequencing of the cloned PCR product is 93 %. c Mutation variations detected in #3 by sequencing analysis. d CAPS analysis of the gDL-3 target locus in regenerated plants obtained from callus line #3. e Variation of mutation detected in regenerated plants. f Phenotypes of dl mutant plants. The bi-allelic mutant plant #3-1 showed the drooping leaves phenotype