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. 2015 Aug 4;6:568. doi: 10.3389/fpls.2015.00568

Figure 1.

Figure 1

Transgenic plants overexpressing PAP5 exhibit enhanced disease susceptibility. (A) Transcript levels of PAP5 in transgenic plants. Total RNA was extracted from wild-type and transgenic plants as described in materials and methods. Transcript levels of PAP5 was normalized to the expression of GAPDH in the same samples and expressed relative to the normalized transcript levels of wild-type plants. The bars represent the mean and standard deviation from two independent experiments. Significant differences (P < 0.05) are indicated by different letters. (B) Growth of Pst DC3000 in wild type and transgenic plants. Plants were inoculated with Pst DC3000 (10-8 c.f.u ml−l) and bacterial growth in plant apoplast was determined as described in the materials and methods. The bars represent the mean and standard deviation from values of three separate trials with six to eight replicates each trial. An asterisk indicates a significance increase in Pst DC3000 growth compared to wild-type plants (Student's t-test; P < 0.05).