Figure 1.

Expression of bHLH factors in NS cells and fluorescent reporter-bHLH fusion constructs. (A) Self-renewing NS cells were immunostained with anti-Sox2, anti-Hes1, anti-Ascl1, and anti-Olig2 antibodies. Hes1, Ascl1, and Olig2 expression levels were variable while another NSC-specific factor, Sox2, was expressed at a relatively constant level. (B) Venus-Hes1 fusion knock-in mouse strain. (C) Venus-Ascl1 fusion BAC Tg mouse strain. (D) mCherry-Olig2 fusion BAC Tg mouse strain. Coding regions of bHLH factors are shown in gray. The pictures and graphs of this figure are reprinted from Supplementary Figures S1C,E; S2A; S6A–D of Imayoshi et al. (2013b).