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. 2015 Aug 4;5:12738. doi: 10.1038/srep12738

Figure 3. USP18 inhibits NF-κB signaling at the level of the IKK complex.

Figure 3

(a) USP18 inhibits NF-κB activation induced by NF-κB pathway downstream signaling molecules. 293T cells were transfected with an NF-κB luciferase reporter, together with vector for MyD88, TAK1-TAB1, TRAF2, TRAF6, IKKα, IKKβ, or p65, along with empty vector (no wedge) or increasing amounts (wedge) of expression vector for USP18. (b) Knockdown of endogenous USP18 enhanced TNF-α, LPS, MyD88, TRAF6, TAK1, and IKKβ induced NF-κB activation. Luciferase activity in HEK293T cells transfected with USP18-specific or scrambled siRNA and transfected with Flag-MyD88, TRAF6, TAK1+TAB1, IKKβ, p65 or treated with TNF-α or LPS (in 293T-TLR4 cells) together with an NF-κB luciferase reporter. Data in a-b are presented as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 versus cells transfected with control vector or scrambled siRNA (Student’s t-test).