Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Aug 4;5:12738. doi: 10.1038/srep12738

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) USP18 overexpression leads to deubiquitination of TAK1. 293T cells were transfected with Fag-TAK1, Myc-USP18, HA-tagged K48 or K63 ubiquitin mutants. Flag-immunoprecipitation was performed and analyzed using anti-HA antibody by immunoblot analysis. (b) USP18 inactive mutants interact with TAK1. 293T cells were transfected with Flag-TAK1 and Myc-USP18 (WT), Myc-USP18 (C64S), or Myc-USP18 (C64S H318A) for 24 hrs. Co-immunoprecipitation and immunoblot analyses were performed with the indicated antibodies. (c) USP18 mutation can not cleave the K63-linked poly-ubiquitin chains on TAK1. Co-immunoprecipitation and immunoblot analysis of 293T cells transfected with Flag-TAK1, HA-K63-ubiquitin and Myc-USP18 (WT), Myc-USP18 (C64S) or Myc-USP18 (C64S H318A).