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. 2015 Aug 4;5:12853. doi: 10.1038/srep12853

Figure 1. LY inhibited RANKL-induced osteoclastogenesis without cytotoxic effects in vitro.

Figure 1

(A) The structure of LY. (B,C) Viability of LY-treated BMMs tested by CCK8 assays at 48 and 96 h. (**P < 0.01). (D) BMMs were treated with various concentrations of LY, M-CSF (30 ng/mL) and RANKL (50 ng/mL) for 5 days. Then, the cells were fixed with 4% paraformaldehyde and stained for TRAP. (E,F) The number and areas of TRAP-positive multinuclear cells were determined as described in the Methods section (*P < 0.05; **P < 0.01).