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Methylcobalamin does not affect the TNF-α-induced apoptosis of SCs. SCs were stimulated with TNF-α for 24 h in the growth medium with or without 100 μM MeCbl. Cleaved caspase-3 (A) and cleaved caspase-9 (B) expression was detected by western blotting. The quantification of the normalized density of cleaved caspase-3 (A) and cleaved caspase-9 (B) is shown. Values are means ± SEM of six independent experiments. *p < 0.05.
