Fig. 3.

Analysis of HPyV12-derived VP1 VLP production in yeast. a - Analysis of HPyV12 VP1³⁸⁰ VLP production in yeast using Coomassie blue-stained SDS-PAGE. Production of HPyV12 VP1³⁸⁰ protein was analyzed in two yeast clones transformed with pFX7-HPyV12-VP1n³⁸⁰ plasmid (lines 1–4) and two clones transformed with pFX7-HPyV12-VP1s³⁸⁰ plasmid (lines 5–8). In lanes: 1, 3 - whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1n³⁸⁰ plasmid; 2, 4 - the soluble fraction recovered after centrifugation of whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1n³⁸⁰ plasmid; 5, 7 - whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1s³⁸⁰ plasmid; 6, 8 - the soluble fraction recovered after the centrifugation of whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1s³⁸⁰ plasmid; 9 - purified HPyV12-VP1³⁸⁰ protein; M - pre-stained protein weight marker. b - Analysis of HPyV12 VP1³⁶⁴ production in yeast using Coomassie blue-stained SDS-PAGE. Production of HPyV12 VP1³⁶⁴ protein was analyzed in two yeast clones transformed with pFX7-HPyV12-VP1n³⁶⁴ plasmid (lines 1–4) and two clones transformed with pFX7-HPyV12-VP1s³⁶⁴ plasmid (lines 5–8). In lanes: 1, 3 - whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1n³⁶⁴ plasmid; 2, 4 - the soluble fraction recovered after centrifugation of whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1n³⁶⁴ plasmid; 5, 7 - whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1s³⁶⁴ plasmid; 6, 8 - the soluble fraction recovered after the centrifugation of whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1s³⁶⁴; 9 - purified HPyV12-VP1³⁶⁴ protein; M - pre-stained protein weight marker (Thermo Fisher Scientific Baltics)