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. 2015 Aug 4;15:68. doi: 10.1186/s12896-015-0187-z

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© Norkiene et al. 2015

Open Access This article is an article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — HA activity of HPyV-derived VP1 VLPs. A 2-fold dilution series of purified KIPyV-, WUPyV-, MCPyV-, HPyV6-, HPyV7-, TSPyV-, HPyV9-, HPyV10-, STLPyV-, HPyV12-, and NJPyV-derived VP1 VLPs expressed in S. cerevisiae were subjected to a HA assay with 1 % guinea pig erythrocytes. Purified JCPyV- and BKPyV-derived VP1 VLPs were used as positive controls, and SV40-derived VP1 VLPs were used as negative controls. The concentrations of VP1-derived VLPs in μg mL⁻¹ are shown on the top. The bar indicates the highest VLP concentration at which HA of guinea pig erythrocytes was observed (HA titer)