Figure 5. Surface representation of Fab D3H44; residues at the interface are colored according to their contribution (in percentage) to (A) the buried surface area (BSA) of Fab upon complex formation and (B) the total number of inter-residue contacts (ICs) made.
Increasing graduation of pink is used for the ranges 0–2%, 2–4%, 4–6%, and above 6% of BSA/ICs contribution. (C) Surface representation of Fab D3H44 (gray) in complex with Tissue factor (light blue), PDB code: 1JPS (Faelber et al., 2001). Fab D3H44 is represented in all panels with the same orientation. Values of residues BSA/ICs contribution are reported in Supplementary file 5. The following figure supplement is available for Figure 5.
Figure 5—figure supplement 1. Comparison between BSA and ICs relative contribution.
(A) Relative contribution (percentage) of each Fab D3H44 interfacial residues to the total BSA (hot pink) and ICs (green).
(B) Corresponding solvent-accessible surface area in Å2 of the Fab D3H44 residues in the free form (separated proteins taken from the complex).