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. 2015 Aug 4;5:12791. doi: 10.1038/srep12791

Figure 1. Schematic illustration of virus infection and TGFβ3 treatment on the palatal shelves organ culture.

Figure 1

Palatal shelves were dissected from E13.5 C57BL/6 mouse embryos and cultured for 3 hours. The palatal shelves were then infected with lentivirus, adenovirus, or treated with 20 ng/ml TGFβ3 for indicated time interval. Then the palatal shelves were harvested for RNA extraction or fixed with 4% PFA or had a change of medium and then were cultured for another 6, 12, 18, or 24 hours, after which they were fixed with 4% PFA.