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. 2015 Aug 4;5:12791. doi: 10.1038/srep12791

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Copyright © 2015, Macmillan Publishers Limited

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Palatal shelves from E13.5 mouse embryos were infected with shLuc, shIrf6 lentivirus, or treated with 20 ng/ml of TGFβ3. (a) Total protein was extracted from palatal shelves at 24 hours after lentivirus infection or TGFβ3 treatment. Expression of IRF6, SNAI2, TWIST, E-cadherin, ZO-1, and Plakophilin protein were examined by Western blotting. GAPDH protein was used as the internal control. (b) Total RNA was extracted from palatal shelves at 24 hours lentivirus infection or TGFβ3 treatment (n = 3). Expression of Irf6, Snai2, and Twist mRNA was analyzed by quantitative RT-PCR. Statistics analysis was performed by t-test. Error bars represent s.d. *p < 0.001; n.s. not significantly different.