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. 2015 Jul 31;197(17):2879–2893. doi: 10.1128/JB.00322-15

FIG 2.

FIG 2

Efficient secretion of XCV0536, XCV3671, XCV4358, and XCV4360 depends on the Xps-T2S system. (A) Efficient secretion of XCV0536, XCV3671, XCV4358, and XCV4360 depends on xpsE and xpsD. Strains 85-10 (wt), 85-10ΔxpsE (ΔxpsE), 85-10ΔxpsD (ΔxpsD), 85-10ΔxcsD (ΔxcsD), and 85-10ΔxpsDΔxcsD (ΔxpsDΔxcsD) without expression constructs (−) or carrying XpsE and/or individual T2S substrates as indicated were incubated in NYG medium. Total cell extracts (TE) and culture supernatants (SN) were analyzed by immunoblotting using a c-Myc epitope-specific antibody. (B) Secretion of the T2S substrates XCV0536, XCV4358, and XCV0965 depends on the N-terminal regions, which contain predicted Sec signal peptides (SP). Strains 85-10 (wt) and 85-10ΔEEE (ΔEEE) carrying full-length XCV0536–c-Myc (XCV0536; FL), XCV0536Δ2–29–c-Myc (XCV0536; ΔSP), XCV4358–c-Myc (XCV4358; FL), XCV4358Δ2–22–c-Myc (XCV4358; ΔSP), XCV0965–c-Myc (XCV0965; FL), or XCV0965Δ2–24–c-Myc (XCV0965; ΔSP) as indicated were incubated in NYG medium. TE and SN were analyzed as described for panel A.