FIG 4.

Venn diagram showing proteins isolated from lysates of S. coelicolor strains expressing gene encoding N-terminally His-tagged Pup. The growth phase at which the pupylated proteins were isolated is indicated by the symbols. The set on the left shows the proteins isolated from a strain with a functional proteasome (Wild-type*). The set on the right shows the proteins isolated from a strain lacking a functional proteasome. The union of the two sets shows the 16 proteins that were captured from the strains with and without a functional proteasome at the same time point(s). As indicated, 2-amino-3-ketobutyrate coenzyme A ligase, tellurium resistance protein, and zinc protease were isolated from both strains, but at different time points.