FIG 1.

Heat map showing levels of transcripts regulated by Anr in PAO1 and J215. RNA from wild-type (WT) PAO1 and J215, and their Δanr derivatives, from duplicate colony biofilms grown for 12 h in 1% O₂ was sequenced. Total reads for each transcript were quantile normalized, log2 transformed, and Z-scored by row. Data for transcripts significantly affected (>2-fold) are shown. Paired columns represent biological replicates. Columns 1 and 2, WT PAO1; columns 3 and 4, PAO1 Δanr; columns 5 and 6, WT J215; columns 7 and 8, J215 Δanr.