FIG 3.

ViuB and YqjH can functionally substitute for each other. V. cholerae (A) or E. coli (B) of the indicated genotypes and carrying the plasmid noted below each set of bars was seeded in EDDA agar, and vibriobactin (Vib), vibriobactin A (VibA), ferrichrome (Fc), enterobactin (Ent), MECAM, or E. coli strain DH5α was spotted on the surface. The zone of growth was measured after 24 h of incubation at 37°C. Each bar represents the average from at least three independent experiments ± standard derivation. The triangles indicate no growth. The strains used in the assay were ALV101 (vibB), JRB9 (viuB), ARM110 (entF), and EWE1026 (entF fes yqjH).