TABLE 1.
Primers used in the HCV strain-specific RT-nested PCR in this study
| Straina and primer type | Primer | Sequence |
|---|---|---|
| H77(1a) | ||
| RT | 2832R-H77 | AAGCGCCCCTAACTTGATGATG |
| PCR I | 2427S-H77 | ACTGGACACGGAGGTGGCCGCGTC |
| 2832R-H77 | AAGCGCCCCTAACTTGATGATG | |
| PCR II | 2462S-H77 | TTGTTCTTGTCGGGTTAATGGCGC |
| 2645R-H77 | GGGTGTACTACACACATGAGTAAG | |
| ED43(4a) | ||
| RT | HCV4aCoseR3591 | CCGTGGTAGACGGTCCACATCAC |
| PCR I | 2676F-ED43 | AGGGCCGGTTCCCAGCTGCT |
| HCV4aCoseR3591 | CCGTGGTAGACGGTCCACATCAC | |
| PCR II | 2839F-ED43 | GCACTACAAGTTATGGCTGGCTA |
| HCV4aCoseR3318 | CACGCAGCGGTGTCAGCGCCCC | |
| SA13(5a) | ||
| RT | 917R-SA13 | CAGTTGCAGTCCTGCACAACATTA |
| PCR I | 741S-SA13 | AGCCCCGAGCCTCGGAGCGGT |
| 917R-SA13 | CAGTTGCAGTCCTGCACAACATTA | |
| PCR II | 748S-SA13 | AGCCTCGGAGCGGTCACGGCT |
| 898R-SA13 | CATTATGCCGGCGAGGGCTAT | |
| HK6a(6a) | ||
| RT | 1765R-HK6a | TGGGGCACAAAAGCTCATTGG |
| PCR I | 1405S-HK6a | TGGGGCCAAATAACCTACAAA |
| 1765R-HK6a | TGGGGCACAAAAGCTCATTGG | |
| PCR II | 1408S-HK6a | GGCCAAATAACCTACAAAGTC |
| 1762R-HK6a | GGCACAAAAGCTCATTGGCAG |
a
Genotypes are given in parentheses.