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. 2015 Jun 17;89(17):9029–9043. doi: 10.1128/JVI.01331-15

FIG 1.

FIG 1

Screening of potential VSR of SARS-CoV by reversal-of-silencing assays. 293T cells were cotransfected with plasmids encoding eGFP reporter (125 ng), eGFP-specific shRNA (shGFP) (1 μg), or luciferase-specific shRNA (shLuc) (1 μg) and viral protein of SARS-CoV (500 ng), respectively. (A to C) The expression level of eGFP was determined by Western blotting at 72 h posttransfection. Empty vector (Vec) and NoV B2 (B2) were used as a mock control and a positive control. The shLuc was used as an irrelevant silencing control. β-Actin was used as a loading control. (D) The expression of SARS-CoV-encoded proteins as indicated was detected by Western blotting. (E) The relative eGFP reversion activity of different viral proteins in panels A to C was normalized by that of typical VSR NoV B2 control and is shown in a bar diagram.