FIG 2.

Oligomannose isomers are the same on PBMC-derived Env and recombinant gp120. Shown are negative-ion CID spectra of oligomannose glycans from the recombinant gp120_JR-CSF. The panels correspond to Man₅GlcNAc₂ (A), Man₆GlcNAc₂ (B), a mixture of Man₇GlcNAc₂ isomers (C), Man₈GlcNAc₂ (D), and Man₉GlcNAc₂ (E). The red dashed lines connect the fragment ions derived from the antenna linked to the 6-position of the core branching mannose residue (termed the 6-antenna), showing their shifts with increasing mannose residues in the 6-antenna of the larger glycans. The images on the right show spectra of the diagnostic region (m/z 450 to 1,000) from the virion-associated gp120 derived from PBMCs, confirming similarity in the glycans within the two samples.