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Plaque morphology of Seg-10 mutants of AHSV4LP. BSR cell monolayers were infected with Seg-10 mutants of AHSV4LP and grown under overlay medium. At 24, 48, and 72 h postinfection, cells were fixed and immunostained. Separate plaques were semiquantitated as having normal CPE (AHSV4LP), small CPE, and no CPE, corresponding to +, small, and −, respectively, in Table 2.
