TABLE 2.
AHSV4LP with mutated Seg-10a
| Virus | CPE detected | IPMA result |
||
|---|---|---|---|---|
| VP2 | NS3 | NS3* | ||
| AHSV4LP | + | + | + | + |
| AHSV4LP without Seg-10 | None | ND | ND | ND |
| mutAUG1 | + | + | + | + |
| mutAUG1+2 | + | + | − | + |
| mutAUG1+2&STOPS | Small | + | − | + |
| delLD | Small | + | − | + |
| delTM1 | − | + | − | − |
| delTM2 | − | + | − | ± |
| delLD&delTM1-2 | − | + | − | − |
a
Rescue of virus without Seg-10 RNA was not successful (none). CPE was normal (+), reduced (small), or absent (−). NS3/NS3a expression was determined by IPMAs of infected monolayers (NS3) and of transient expression (NS3*) as positive (+), very weak (±), or negative (−). ND, not determined.