TABLE 3.
AHSV4LP with exchanged Seg-2 and mutated Seg-10 (AHS DISA vaccine candidates)a
| Virus | Seg-2 serotype | Segment of AHSV4LP | CPE | VP2 serotype determined by IPMA | NS3 expression determined by IPMA |
|---|---|---|---|---|---|
| AHSV4LP | 4 | 1–10 | + | 4 | + |
| mutAUG1+2 | 4 | 1–9 | + | 4 | + |
| mutAUG1+2&STOPS | 4 | 1–9 | Small | 4 | − |
| AHSV1LP-(NS3/NS3a)mut | 1 | 1, 3–9 | Small | 1 | − |
| AHSV8LP-(NS3/NS3a)mut | 8 | 1, 3–9 | Small | 8 | − |
a
Data for the mutation mutAUG1+2&STOPS (NS3/NS3amut) in combination with Seg-2 exchange in AHSV1LP-(NS3/NS3a)mut and AHSV8LP-(NS3/NS3a)mut are shown. CPE was normal (+) or reduced (small). Expression was determined by IPMA as positive for the indicated serotype for VP2 and as positive (+) or negative (−) for NS3.