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HNIgGD5 and HNIgGH8 are able to neutralize H7N9 virus. (A) The binding activity of the antibodies to HA and NA proteins were determined by IFA. (B) Neutralizing activities of HNIgGD5 and HNIgGH8 against H7N9 virus were tested on MDCK cells. An irrelevant human IgG was utilized as a control. (C) Epitope mapping of the antibodies. The selected amino acids in H7N9 HA were converted to the residues indicated. The mutated HA protein was then transiently expressed in cells and detected by IFA.
