FIG 2.

Validation of PP1 catalytic subunits PARD3 and RASSF8 as E4orf4 binding partners. HA-E4orf4 species were immunoprecipitated (IP) using anti-HA antibodies, and Western blotting was performed using appropriate anti-FLAG or anti-MYC antibodies. (A) Binding of FLAG-PP1 catalytic subunits (PP1 Cα, PP1 Cβ, and PP1 Cγ) or FLAG-GFP as a negative control. Whole-cell extracts (WCE) are shown for FLAG-GFP/PP1 catalytic species (PP1 Cα, PP1 Cβ, and PP1 Cγ) and HA-E4orf4 species (E4orf4 wild type and class I mutant R81A/F84A). (B) PARD3. (C) RASSF8.