FIG 7.

E4orf4 cell killing in control and YAP1 knockdown cells. Cell killing was measured by colony formation assays in control H1299 cells and those in which YAP1 expression was reduced by the expression of an appropriate shRNA following expression of wild-type E4orf4, as described in Materials and Methods. (Top and middle) Effect on colony formation. (Bottom) YAP1 and HA-E4orf4 expression as measured by Western blotting. Error bars represent standard deviations, and the P value was calculated from a t test with two paired samples for means.