FIG 10.
Characterization of sh-Luc-HEp-2, sh-p32-HEp-2, sh-p32-HEp-2/Ct, and sh-p32-HEp-2/p32(+) cells. (A) Expression of p32 in sh-Luc-HEp-2 and sh-p32-HEp-2 cells analyzed by immunoblotting with anti-p32 (top) and anti-β-actin (bottom) antibodies. (B) Cell viability of sh-Luc-HEp-2 and sh-p32-HEp-2 cells assayed 24 h after 2 × 104 cells were seeded on 96-well plates. Each value is the mean ± standard error of the results of triplicate experiments and is expressed relative to the mean for sh-Luc-HEp-2 cells, which was normalized to 100%. n.s., not statistically significant. Data are representative of three independent experiments. (C) Expression of p32 in sh-p32-HEp-2/Ct and sh-p32-HEp-2/p32(+) cells analyzed by immunoblotting with anti-p32 (top) and anti-β-actin (bottom) antibodies.