FIG 2.

Identification of cellular proteins interacting with HSV-1 UL47. (A) Schematic diagram of expression plasmid pcDNA-MEF-UL47 encoding UL47 fused to an MEF tag. (B and C) 293T cells were transfected with the empty vector pcDNA-MEF or plasmid pcDNA-MEF-UL47, harvested, and immunoprecipitated with anti-Myc antibody and anti-Flag antibody. Immunoprecipitates were separated in 7.5% (B) or 12% (C) denaturing gels and silver stained. Bands marked with asterisks were excised, digested, and analyzed by mass spectrometry. The arrow marks MEF-UL47 and p32. Molecular mass markers are indicated on the left.