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. 2015 Jun 17;89(17):8982–8998. doi: 10.1128/JVI.01220-15

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FIG 3 — Interaction of UL47 with p32. (A) 293T cells were mock transfected or transfected with pcDNA-MEF-UL47 alone, pCMV-p32(F) encoding Flag-tagged p32 alone, or pCMV-p32(F) in combination with either pcDNA-MEF-UL47 or pcDNA-MEF-gB. At 2 days posttransfection, cells were harvested, immunoprecipitated (IP) with anti-Myc antibody, and analyzed by immunoblotting (IB) with anti-Flag antibody. WCE, whole-cell extract. Molecular mass markers are indicated on the left. (B) Vero cells infected with wild-type HSV-1(F) or YK536 (MEF-UL47) at an MOI of 5 for 18 h were harvested, immunoprecipitated with anti-Flag antibody, and analyzed by immunoblotting with anti-p32 antibody, anti-ICP8 antibody, or anti-Flag antibody.