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Effect of Us3 kinase activity on localization of p32, UL31, and UL34 in HSV-1-infected cells. Vero cells were infected with wild-type HSV-1(F), YK511 (Us3K220M), or YK513 (Us3K220M-repair) at an MOI of 3, fixed at 18 h postinfection, permeabilized, stained with anti-p32 antibody in combination with anti-UL34 (A) or anti-UL31 (B) antibody, and examined by confocal microscopy. Scale bar, 5 μm.
