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Effect of Us3 kinase activity on localization of p32 and mRFP1-UL47 in HSV-1-infected cells. Vero cells were infected with YK524 (mRFP1-UL47), YK527 (mRFP1-UL47/Us3K220M), or YK528 (mRFP1-UL47/Us3K220M-repair) at an MOI of 3, fixed at 18 h postinfection, permeabilized, stained with anti-p32 antibody, and examined by confocal microscopy. Scale bar, 5 μm.
