FIG 8.

Localization of p32 in HSV-1-infected cells by immunoelectron microscopy. Vero cells were infected with wild-type HSV-1(F) at an MOI of 5, fixed at 18 h postinfection, embedded, sectioned, stained with rabbit anti-p32 polyclonal antibody followed by goat anti-rabbit IgG conjugated to 10-nm gold particles, and examined by transmission electron microscopy. Nu, nucleus; Cy, cytoplasm; NM, nuclear membrane; INM, inner nuclear membrane; ONM, outer nuclear membrane; PM, plasma membrane. p32 was detected along the nuclear membrane (A), on capsids in the nucleus (A to C and H) and cytoplasm (E and I), and on primary enveloped virions in the perinuclear space (C and D), but it was barely detectable on secondary enveloped virions in the cytoplasm (F and I) and in the extracellular space (G and J). Bars, 200 nm.