TABLE 1.
Effect of p32 knockdown on distribution of progeny virus particles in infected HEp-2 cells
| Cell type | Virus | No. of intranuclear invaginations | No. of virus particles in morphogenetic stagea |
Total counted (particles/cells) | |||||
|---|---|---|---|---|---|---|---|---|---|
| Nucleocapsids in the nucleus | Enveloped virions in intranuclear invaginations | Enveloped virions in the perinuclear space | Nucleocapsids in the cytoplasm | Enveloped virions in the cytoplasm | Extracellular enveloped virions | ||||
| sh-Luc-HEp-2 | HSV-1 (F) | 1 | 639 (22.3) | 4 (0.1) | 372 (13.0) | 228 (7.9) | 678 (23.7) | 947 (33.0) | 2,868/22 |
| sh-Luc-HEp-2 | YK545 (ΔUL47) | 0 | 600 (56.4) | 0 (0) | 22 (2.1) | 97 (9.1) | 46 (4.3) | 299 (28.1) | 1,064/22 |
| sh-Luc-HEp-2 | YK546 (ΔUL47-repair) | 0 | 755 (28.9) | 0 (0) | 285 (10.9) | 210 (8.0) | 563 (21.5) | 801 (30.7) | 2,813/22 |
| sh-p32-HEp-2 | HSV-1 (F) | 21 | 607 (30.5) | 101 (5) | 295 (14.8) | 268 (13.5) | 324 (16.3) | 396 (19.9) | 1,991/22 |
| sh-p32-HEp-2 | YK545 (ΔUL47) | 0 | 449 (61.8) | 0 (0) | 0 (0) | 71 (9.8) | 55 (7.6) | 151 (20.8) | 726/22 |
| sh-p32-HEp-2 | YK546 (ΔUL47-repair) | 17 | 552 (29.4) | 103 (5.5) | 181 (9.7) | 274 (14.6) | 366 (19.5) | 400 (21.3) | 1,876/22 |
a
Numbers in parentheses are the percentages of virus particles in the morphogenetic stage.