TABLE 2.
Effect of p32 knock-in on distribution of progeny virus particles in HSV-1(F)-infected HEp-2 cells
| Cell type | No. of intranuclear invaginations | No. of virus particles in morphogenetic stagea |
Total counted (particles/cells) | |||||
|---|---|---|---|---|---|---|---|---|
| Nucleocapsids in the nucleus | Enveloped virions in intranuclear invaginations | Enveloped virions in the perinuclear space | Nucleocapsids in the cytoplasm | Enveloped virions in the cytoplasm | Extracellular enveloped virions | |||
| sh-p32-HEp-2/Ct | 23 | 616 (31.5) | 130 (6.7) | 234 (12.0) | 257 (13.1) | 314 (16.0) | 406 (20.7) | 1,957/22 |
| sh-p32-HEp-2/p32(+) | 2 | 633 (23.3) | 7 (0.3) | 345 (12.7) | 199 (7.4) | 655 (24.1) | 874 (32.2) | 2,713/22 |
a
Numbers in parentheses are the percentages of virus particles in the morphogenetic stage.