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. 2015 Jul 2;35(15):2658–2672. doi: 10.1128/MCB.00161-15

FIG 4.

FIG 4

HIF2α is essential for the development of polycythemia in liver-specific PHD knockout mice. (A) Hematocrit levels in PHD and HIF2α mutant mice at 8 to 16 weeks of age are shown as the means and SD (n = 3 to 5 for each group). *, P < 0.01 compared with control H2-LKO mice. (B) Immunoblots for HIF2α in nuclear extracts from the livers of PHD and HIF2α mutant mice at 4 to 10 weeks of age. Nup62 was used as an internal control. The data from 2 independent samples are shown for each genotype group. (C) mRNA expression levels in the livers of PHD and HIF2α mutant mice were analyzed by RT-qPCR. β-Actin was used as an internal control for RT-qPCR. The expression levels of control mice were set as 1.0. The data are means and SD (n = 3 for each group). *, P < 0.01 compared with the control mice (white bar). Note that the overexpression of Epo mRNA in P123-LKO livers is attenuated by the loss of HIF2α (P123H2-LKO).