TABLE 1.
Sequences of the primers used in this study
| Purpose and target | Primer 1 (5′–3′) | Primer 2 (5′–3′) |
|---|---|---|
| Genotyping | ||
| Egln1 (PHD2) | CAAATGGAGATGGAAGATGC | TCAACTCGAGCTGGAAACC |
| Egln2 (PHD1) | TGGGCGCTGCATCACCTGTATCT | ACTGGTGAAGCCTGTAGCCTGTC |
| Egln3 (PHD3) | ATGGCCGCTGTATCACCTGTAT | CCACGTTAACTCTAGAGCCACTGA |
| AlbCre | ACGTTCACCGGCATCAACGT | CTGCATTACCGGTCGATGCA |
| Epas1 (HIF2α) | CAGGCAGCAGGCAGTATGCCTGGCTAATTCCAGTT | CTTCTTCCATCATCTGGGATCTGGGACT |
| EpoHE | CAGGCTCCATTCAAGGC | CCTGCAGTGGACTTTGAAGGC |
| RT-PCR with gel electrophoresis | ||
| PHD1 | ACCGCGCAGCATTCGTG | GGGGCTGGCCATTAGGTAGGTGTA |
| PHD2 | GCGGGAAGCTGGGCAACTAC | CAACCCTCACACCTTTCTCACC |
| PHD3 | CTGCGTGCTGGAGCGAGTCAA | TCATGTGGATTCCTGCGGTCTG |
| IPAS/HIF3α | GAGGGTTTCGTCATGGTACT | TCTTGAAGTTCCTCTTGGTC |
| RT-qPCR with SYBR green reagents for mouse (m) or human (h) mRNA expression | ||
| β-Actin (m, h) | GACAGGATGCAGAAGGAGAT | TTGCTGATCCACATCTGCTG |
| PHD1 (m) | ATGGCTCACGTGGACGCAGTAA | CATTGCCTGGATAACACGCCAC |
| PHD2 (m) | TAAACGGCCGAACGAAAGC | GGGTTATCAACGTGACGGACA |
| PHD3 (m) | CTATGTCAAGGAGCGGTCCAA | GTCCACATGGCGAACATAACC |
| Epo (m) | CATCTGCGACAGTCGAGTTCTG | CACAACCCATCGTGACATTTTC |
| EpoR (m) | GGGCTCCGAAGAACTTCTGTG | ATGACTTTCGTGACTCACCCT |
| Bnip3 (m) | GTTACCCACGAACCCCACTTT | GTGGACAGCAAGGCGAGAAT |
| Vegfa (m) | CTGCTGTAACGATGAAGCCCTG | GCTGTAGGAAGCTCATCTCTCC |
| Fasn (m) | GGCTGCTGTTGGAAGTCAGC | AGTGTTCGTTCCTCGGAGTG |
| Scd1 (m) | TTCTTGCGATACACTCTGGTGC | CGGGATTGAATGTTCTTGTCGT |
| HIF1α (m) | CCTGCACTGAATCAAGAGGTTGC | CCATCAGAAGGACTTGCTGGCT |
| HIF2α (m) | CAATGACAGCTGACAAGGAG | CATAGAAGACCTCCGTCTCC |
| HIF3α (m) | AAGACGCCCTGACCCCCAGG | CCCTCTGCTGGTGAGCGTGC |
| Gpi (m) | CCCTCTTTATAATCGCCTCCA | GAAACCACTCCTTTGCTGTCTC |
| Ldha (m) | GGCACTGACGCAGACAAG | TGATCACCTCGTAGGCACTG |
| Hepcidin (m) | TCTTCTGCATTGGTATCGCA | GAGCAGCACCACCTATCTCC |
| CA9 (h) | CCTTTGCCAGAGTTGACGAG | GACAGCAACTGCTCATAGGC |
| PGK1 (h) | CTAACAAGCTGACGCTGGAC | CTGGTTGTTTGTTATCTGGTTG |
| EPO (h) | TCATCTGTGACAGCCGAGTC | CAAGCTGCAGTGTTCAGCAC |
| qPCR with SYBR green reagents for chromatin analysis | ||
| CA9 promoter | TCTGTGAGTCAGCCTGCTCC | TCCCAGCACACGGTGTGTAC |
| PGK1 promoter | CCCTAAGTCGGGAAGGTTCC | CTGTCCGTCTGCGAGGGTAC |
| EPO promoter | CTCAACCCAGGCGTCCTG | GGGGCTGTTATCTGCATGTG |
| EPO intron1 | CTGTTTGAGCGGGGATTTAGC | TTCCGGGGTCCTTGACAAGT |
| EPO HE | AACCTCCAAATCCCCTGGCTC | CTGTGTGAGACAGCACGTAG |