FIG 7.

The Rax protein physically associates with the Crx protein. (A to C) Rax colocalizes with Crx in the postnatal retina. (A and B) At P4, retinal sections were immunostained with anti-Rax (magenta) (A) and anti-Crx (green) (B) antibodies. (C) Merge of panels A and B. (Inset) Rax- and Crx-double-positive cells at high magnification. Bar, 50 μm. (D and E) The Rax protein interacts with the Crx protein. The HA-tagged Rax expression plasmid was transfected with the FLAG-tagged Crx expression plasmid into HEK293T cells. Cell lysates were incubated with either an anti-FLAG antibody (D) or an anti-HA antibody (E). Immunoprecipitated proteins were analyzed by Western blotting. Lanes 5 to 8, input proteins without immunoprecipitation (IP). Arrow, protein immunoprecipitated by the anti-FLAG antibody; arrowhead, protein immunoprecipitated by the anti-HA antibody. IB, immunoblot; NBL, neuroblastic layer.