TABLE 1.
Primer sequences used for qPCR analysis
| Gene | Orientation | Primer sequence (5′-3′) |
|---|---|---|
| M-opsin | Sense | TTGCTTTGCCACTGCTCG |
| Antisense | CTGTGGCCCAGACGTGTTC | |
| S-opsin | Sense | GCTGGACTTACGGCTTGTCACC |
| Antisense | TGTGGCGTTGTGTTTGCTGC | |
| Gnat2 | Sense | ATGCTGACAAGGAAGCCAAGACTG |
| Antisense | GACAGACTTGAACTCTAGGCACTC | |
| Pde6c | Sense | GATCAACGTGATTCCGTCACCTCC |
| Antisense | AGTCTCATCTACCGGTCCTTTCTG | |
| Crx | Sense | CACCAGGCTGTCCCATACTC |
| Antisense | TCGCCCTACGATTCTTGAAC | |
| Rcvrn | Sense | GAGTACGTGATTGCTCTGCACATG |
| Antisense | AGGAGTTTCACATCCTCAGGCTTG | |
| Sag | Sense | AGCCTGCTCAAGAAACTGGGAGACA |
| Antisense | AGCTGGCTGCAACATCACTGAACA | |
| Nrl | Sense | GCTGTGCCTTTCTGGTTCTGA |
| Antisense | GCTCCCGCTTTATTTCGAACT | |
| Nr2e3 | Sense | TTGATGTCACCAGCAATGACC |
| Antisense | TTTTGGCCCATTTGACAGC | |
| Rhodopsin | Sense | GCTTCCCTACGCCAGTGTG |
| Antisense | CAGTGGATTCTTGCCGCAG | |
| Gnat1 | Sense | CGCTCAACATTCAGTATGGAGA |
| Antisense | CGAAGTAGCGATGATTGCAGATG | |
| Pde6g | Sense | CAAACAAGGCAGTTCAAGAGCAAG |
| Antisense | CAGGGATCTGACTAAATGATGCCA | |
| Gapdha | Sense | ACTGGCATGGCCTTCCGTGTTCCTA |
| Antisense | TCAGTGTAGCCCAAGATGCCCTTC |
a
Gapdh, glyceraldehyde-3-phosphate dehydrogenase gene.