FIG 1.

IFN-α-induced expression of SLFN genes in human renal cell carcinoma cells. (A) 786-0 cells were treated with IFN-α for the indicated time. Total mRNA was isolated, and expression of SLFN5, SLFN11, SLFN12, and SLFN13 was measured by real-time RT-PCR, using GAPDH as a housekeeping gene. Data are expressed as the fold increase over control untreated samples and represent means ± standard errors of results from four independent experiments. Paired two-tailed t test analysis showed a two-tailed P value of 0.0108 for SLFN5 at 6 h compared to control. (B) 786-0 cells were treated with IFN-α as indicated, and after cell lysis, total proteins were resolved by SDS-PAGE and immunoblotted with an anti-SLFN5 antibody or an anti-GAPDH antibody. (C) ACHN cells were treated with IFN-α for the indicated times. Total RNA was isolated, and expression of SLFN5, SLFN11, SLFN12, SLFN13, and SLFN14 was measured by real-time RT-PCR using GAPDH as a housekeeping gene. Data are expressed as the fold increase over control of untreated samples and represent means ± standard errors of results from three independent experiments. Paired two-tailed t test analysis showed a two-tailed P value of 0.0188 for SLFN5 at 6 h compared to control. (D) ACHN cells were treated with IFN-α as indicated, and after cell lysis, proteins were resolved by SDS-PAGE and immunoblotted with an anti-SLFN5 antibody or an anti-α tubulin antibody.