TABLE 3.
Specificity of the O121 wzx (O-antigen flippase) real-time PCR within strains belonging to serogroup O121
| Serotypea | No. of strains | Country of isolation and date (yr) or reference | Sample typeb | STEC virulence gene(s)c |
|---|---|---|---|---|
| O121:H7 | 1 | Germany, 2008 | Wild boar meat | stx1, stx2 |
| O121:H9 | 1 | Germany, 2014 | Raw sausage | stx2e |
| O121:H10 | 2 | Germany, 2011 and 2014 | Raw sausage | stx2e |
| O121:H10 | 4 | Germany, 2007–2013 | Pig feces (n = 2), ground meat (n = 1), pig organ (n = 1) | |
| O121:H11 | 1 | Germany, 2009 | Calf feces | |
| O121:H14 | 1 | Germany, 2008 | Red deer meat | stx1c |
| O121:H19 | 1 | France (6) | ||
| O121:H19 | 2 | Switzerland (25); Germany, 2014 | Sheep raw milk | eae |
| O121:H19 | 13 | Germany (23); Switzerland (25); France (6) | stx2, eae | |
| O rough:H19d | 1 | Switzerland (25) | stx2, eae |
a
All strains reacted with the O121 wzx real-time PCR. Some of these strains were nonmotile, and their fliC genotype was detected by nucleotide sequencing of fliC PCR products.
b
n, number of samples.
c
Presence of stx and eae genes as major markers of EHEC. In brackets: numbers of strains with corresponding genotype.
d
This O-rough strain gave a positive result in the O121 wzx real-time PCR.