FIG 2.
Rotavirus infectivity after solar and 57°C treatments. The infectivity data from at least two independent experiments were aggregated and plotted here as log10(FFUt/FFU0) versus treatment time (hours). Inactivation experiments were conducted in solutions containing 1 mM NaHCO3 buffer (pH 8), 0 or 20 mg C/liter of an organic sensitizer, and an initial rotavirus concentration of 2 × 105 FFU/ml. Samples of treated or untreated rotaviruses were inoculated onto MA104 cell monolayers. At 15 to 18 h postinfection, cells were fixed and immunostained, and FFUs were quantified using an inverted microscope.