TABLE 5.
Antifungal polypeptides identified by nano-LC–ESI–MS-MS in the water/salt-soluble extract from the pea flour hydrolysatea after 6 h of incubation at 30°C
| Pisum sativum protein | NCBI accession no. | Sequenceb | Theoretical mass (kDa) | Coverage (%) | Mascot score |
|---|---|---|---|---|---|
| Defensin-1 | P81929 | KTCEHLADTYRGVCFTNASCDDHCKNKAHLISGTCHNWKCFCTQNC | 5.21 | 82 | 55 |
| Defensin-2 | P81930 | KTCENLSGTFKGPCIPDGNCNKHCRNNEHLLSGRCRDDFRCWCTNRC | 5.40 | 75 | 54 |
| Nonspecific lipid transfer protein | O24309 | MVVVSAPMAEAAISCGAVTAAVAPCFAYLKGAPSPSLQCCGGVRRLNGIPDRKGVCNCLKGAAGSVPGLKPGVAALPAN VVLDFLSRLA PLPTVMPSVFKKKCG | 10.49 | 60 | 65 |
a
Obtained with a neutral protease from Aspergillus oryzae (Veron PS; dosage, 25 g/100 kg of protein).
b
Single-letter amino acid code is used.