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MNV-1 titer associated with A. castellanii and A. polyphaga 1 h after virus-amoeba inoculation at virus/amoeba ratio 1:1. The input virus value was 6.47 log10 (3 × 106 PFU), and the recovered virus value means from A. castellanii and A. polyphaga were 2.69 log10 (490 PFU) and 2.52 log10 (337 PFU), respectively. Values are means ± the standard errors from at least three experiments.*, P < 0.01 versus the amoeba control.
