FIG 3.

Plasmids for heterologous expression of xylEFGD genes in C. glutamicum. (A) Architecture of expression cassettes on xylD-harboring plasmid pCRF100, xylEFGD-harboring plasmid pCRF101, xylEFGD- and cgR_2369-harboring plasmid pCRF102, and xylEFGD- and msiK-harboring plasmid pCRF103. All plasmid genes are under the control of the strong constitutive tac promoter. Each expression cassette is on the C. glutamicum-E. coli shuttle vector where the corynebacterial replication of origin is from plasmid pBY503. (B) Secondary structure of the translation initiation region of the xylD gene as predicted by the RNAfold WebServer (http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi).