FIG 6.

Detection of intracellular arabino-xylooligosaccharides. Strain X1EFGD was first grown aerobically to late log phase in A medium containing 40 g liter⁻¹ glucose and then inoculated to an initial OD₆₁₀ of 0.5 into A medium containing 2.5 g liter⁻¹ xylooligosaccharide mixture. (A) Culture medium analyzed by LC-ESI-MS at 0 h. (B) Culture medium analyzed by LC-ESI-MS at 24 h. (C) Cell extract analyzed by LC-ESI-MS at 24 h. For each analysis, negative ions corresponding to pentose oligosaccharides composed of 4 to 6 pentose units were detected. Compounds P4, P5, and P6 were oligosaccharides whose molecular weights corresponded to pentose oligosaccharides composed of 4 to 6 pentose units. X4, xylotetraose.