TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| HST02 | F′ [traD36 proA+B+ lacIq lacZΔM15] D(lac-proAB) recA endA1 gyrA96 thi e14 mutant (mcrA mutant), supE44 relA1 ΔdeoR Δ(mrr-hsdRMS-mcrBC) | TaKaRa |
| SCS110 | rpsL (Strr) thr leu endA thi-1 lacY galK galT ara tonA tsx dam dcm supE44 Δ(lac-proAB) [F′ traD36 proAB lacIqZΔM15] | Agilent |
| BL21(DE3) | F− ompT hsdSB(rB− mB−) gal dcm (DE3) | TaKaRa |
| C. alkanolyticum ATCC 21511 | ||
| C. glutamicum | ||
| R | JCM18229 | 17 |
| X1 | Markerless Ptrc-xylA and Ptrc-xylB gene recombinant integrated into Xyl1 of strain R | 9 |
| X1D | Kmr; C. glutamicum X1 bearing pCRF100 | This work |
| X1EFGD | Kmr; C. glutamicum X1 bearing pCRF101 | This work |
| X1(Δ2369) | C. glutamicum X1 ΔcgR_2369 | This work |
| X1(Δ2369)D | Kmr; C. glutamicum X1Δ2369 bearing pCRF100 | This work |
| X1(Δ2369)EFGD | Kmr; C. glutamicum X1Δ2369 bearing pCRF101 | This work |
| X1(Δ2369)EFGDK | Kmr; C. glutamicum X1Δ2369 bearing pCRF102 | This work |
| X1(Δ2369)EFGDM | Kmr; C. glutamicum X1Δ2369 bearing pCRF103 | This work |
| BsrAXH | Kmr; C. glutamicum R bearing pCRF104 | This work |
| Plasmids | ||
| pCRB52T | Kmr; Ptac promoter; E. coli-Corynebacterium sp. shuttle vector derived from pHSG298 and pBY503 | This work |
| pCRF100 | Kmr; pCRB52T with a 2.4-kb NdeI-KpnI PCR fragment of the xylD gene | This work |
| pCRF101 | Kmr; pCRB52T with a 5.6-kb NdeI-KpnI PCR fragment of the xylEFGD gene cluster | This work |
| pCRF102 | Kmr; pCRF101 with a 1.1-kb KpnI PCR fragment of the cgR_2369 gene | This work |
| pCRF103 | Kmr; pCRF101 with a 1.1-kb KpnI PCR fragment of the msiK gene | This work |
| pCRF104 | Kmr; pCRA725 with a 3.0-kb XbaI PCR fragment of the target sequence for deletion of the cgR_2369 gene | This work |
| pCRA725 | Kmr; pHSG298 with Ptac-sacR-sacB genes | |
| pCRF105 | Kmr; pCRB52T with a 1.5-kb NdeI-BglII PCR fragment of the axh gene | This work |
| pCRF106 | Amr; pColdII with a 2.4-kb NdeI-BglII PCR fragment of the xylD gene | This work |