TABLE 2.
Oligonucleotides used in this study
| Primer name | Sequence (5′–3′)a | Cohesive end |
|---|---|---|
| DPXF | CACGAGGA(A/G)TG(C/G)CT(C/G)(A/G)C(C/G)GG | |
| DPXR | CG(G/C)TG(G/C)GA(G/C)TT(T/C)GTGAAGC(G/A)G | |
| DPMF | CG(C/G)GACATCGC(A/C)ATGGT(G/T)TTCCAGA | |
| DPMR | CAGCG(A/G)CCGAAGTAGCCGAG | |
| APF | TCCGAGCGTTGCATGACCACAGTAAGCTATCACGTAC | |
| AP-NotIR | GGCCGTACGTGATAGCTTAC | |
| AP-BamHIR | GATCGTACGTGATAGCTTAC | |
| AP-MluIR | CGCGGTACGTGATAGCTTAC | |
| A P1 | CCGAGCGTTGCATGACCACAGTAAGCT | |
| AP2 | CGTTGCATGACCACAGTAAGCTATCACGTA | |
| GWR1-R2 | CGCCCATCTCGGCGACGAGGTC | |
| GWR1-R1 | AGCCCCTGGTGGATCCCGAGCTC | |
| GWF1-F2 | CGTCGACGAGTGCATCGCCGAGGA | |
| GWF1-F1 | GCCGCGATCGGCGACACGATGC | |
| GWR2-R2 | CGATTGAAACACGGCCGTGTCCGACC | |
| GWR2-R1 | GCGAAGACGAGAATGTCATCGCTCAC | |
| CAmsi-R2 | GGCGATCTTGAGCGCGAAGCCCATG | |
| CAmsi-R1 | ACCCGAGAGGGCCTTCGGCTTGC | |
| CAmsi-F2 | CGACCGCATCGCGGTCCTCAAGGA | |
| CAmsi-F1 | CCTCGGCGTCACCACGGTCTACG | |
| Primer 1 | GCCGGCAACCATATGACCGAACTGCCCCCTCT | NdeI |
| Primer 2 | GGGGTACCTCAGCTGACGGGTGCCTCGGCC | KpnI |
| Primer 3 | GGGAATTCCATATGAGAGCACACAGGATCCTGACG | NdeI |
| Primer 4 | GGGGTACCACGGATCGTTCGGCACGTAC | KpnI |
| Primer 5 | GGGGTACCTTAAGGGAGGCTCTTGCCAG | KpnI |
| Primer 6 | GCGGTACCACTACGGATCGTCCGGCACGTA | KpnI |
| Primer 7 | CCGGTACCTTACGCCGAGACGATCGCCTTG | KpnI |
| Primer 8 | GGAATTCCATATGAGGAAAAAGTGTAGCGTATGTTTA | NdeI |
| Primer 9 | GAAGATCTCTATCTTTGCGTAAACTGCCAG | BglII |
| Primer 10 | AGGAAGATCTCAGCTGACGGGTGCCTCGGCC | BglII |
| CgR2369_d1F | GCTCTAGAAAGGAACATCGTCATCGACCTTG | XbaI |
| CgR2369_d1R | GACAAAGCCCGGAAGCCATG | |
| CgR2369_d2F | CATGGCTTCCGGGCTTTGTCCTACCCCGAGGCACGTAATG | |
| CgR2369_d2R | GCTCTAGAGGCGGCAGTAACGACCTTGAC | XbaI |
a
The restriction site overhangs used in the cloning procedure are underlined.