FIG 4.

(A) Relative transcript levels of benA, encoding the initial step of benzoate (Bz) degradation by Cupriavidus pinatubonensis JMP134, exposed to binary Bz-containing mixtures of aromatic compounds (AC) or single AC. (B) Expanded data for single AC other than Bz. Fructose-grown cells (OD₆₀₀ = 0.7) were exposed to the binary mixture (a 5 mM concentration of each AC, except 2 mM phenol) or the single AC (5 mM, except 2 mM phenol) for 1 h. Values (three biological and two technical replicates) correspond to the average and standard error, normalized with respect to the rps7 housekeeping reference gene. Different letters indicate statistically significant differences between values (one-way ANOVA and Tukey's HSD test; P < 0.05).